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+PerlPrimer is a free, open-source GUI application written in Perl
+that designs primers for standard PCR, bisulphite PCR, real-time PCR
+(QPCR) and sequencing. It aims to automate and simplify the process
+of primer design.
+
+PerlPrimer's current features include the following:
+
+- Calculation of possible primer-dimers
+- Retrieval of genomic or cDNA sequences from Ensembl (including both
+ sequences automatically for QPCR)
+- Ability to BLAST search primers using the NCBI server or a local
+ server
+- Results can be saved or optionally exported in a tab-delimited
+ format that is compatible with most spreadsheet applications.
+- ORF and CpG island detection algorithms
+- Ability to add cloning sequences to primers, automatically adjusted
+ to be in-frame
+- QPCR primer design without manual intron-exon boundary entry
+
+For details and citation:
+Marshall OJ. PerlPrimer: cross-platform, graphical primer design for
+standard, bisulphite and real-time PCR. Bioinformatics 2004
+20(15):2471-2472
+
+This requires perl-tk and libwww-perl.
+For Real Time PCR, spidey is required.