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diff --git a/academic/perlprimer/README b/academic/perlprimer/README new file mode 100644 index 000000000000..12917e35e92a --- /dev/null +++ b/academic/perlprimer/README @@ -0,0 +1,26 @@ +PerlPrimer is a free, open-source GUI application written in Perl +that designs primers for standard PCR, bisulphite PCR, real-time PCR +(QPCR) and sequencing. It aims to automate and simplify the process +of primer design. + +PerlPrimer's current features include the following: + +- Calculation of possible primer-dimers +- Retrieval of genomic or cDNA sequences from Ensembl (including both + sequences automatically for QPCR) +- Ability to BLAST search primers using the NCBI server or a local + server +- Results can be saved or optionally exported in a tab-delimited + format that is compatible with most spreadsheet applications. +- ORF and CpG island detection algorithms +- Ability to add cloning sequences to primers, automatically adjusted + to be in-frame +- QPCR primer design without manual intron-exon boundary entry + +For details and citation: +Marshall OJ. PerlPrimer: cross-platform, graphical primer design for +standard, bisulphite and real-time PCR. Bioinformatics 2004 +20(15):2471-2472 + +This requires perl-tk and libwww-perl. +For Real Time PCR, spidey is required. |