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Diffstat (limited to 'academic/cufflinks')
-rw-r--r-- | academic/cufflinks/README | 17 | ||||
-rw-r--r-- | academic/cufflinks/References | 37 | ||||
-rw-r--r-- | academic/cufflinks/cufflinks.SlackBuild | 107 | ||||
-rw-r--r-- | academic/cufflinks/cufflinks.info | 10 | ||||
-rw-r--r-- | academic/cufflinks/slack-desc | 19 |
5 files changed, 190 insertions, 0 deletions
diff --git a/academic/cufflinks/README b/academic/cufflinks/README new file mode 100644 index 0000000000000..7afb95ee6e87d --- /dev/null +++ b/academic/cufflinks/README @@ -0,0 +1,17 @@ +Cufflinks assembles transcripts, estimates their abundances, and tests +for differential expression and regulation in RNA-Seq samples. It +accepts aligned RNA-Seq reads and assembles the alignments into a +parsimonious set of transcripts. Cufflinks then estimates the relative +abundances of these transcripts based on how many reads support each +one, taking into account biases in library preparation protocols. + +Cufflinks is a collaborative effort between the Laboratory for +Mathematical and Computational Biology, led by Lior Pachter at UC +Berkeley, Steven Salzberg's computational genomics group at the +Institute of Genetic Medicine at Johns Hopkins University, and Barbara +Wold's lab at Caltech. + +Reference: +Ali Mortazavi, Brian A Williams, Kenneth McCue, Lorian Schaeffer and +Barbara Wold, "Mapping and quantifying mammalian transcriptomes by +RNA-Seq", Nature Methods, volume 5, 621 - 628 (2008) diff --git a/academic/cufflinks/References b/academic/cufflinks/References new file mode 100644 index 0000000000000..3434915871c64 --- /dev/null +++ b/academic/cufflinks/References @@ -0,0 +1,37 @@ +Publications + +Trapnell C, Williams BA, Pertea G, Mortazavi AM, Kwan G, van Baren MJ, +Salzberg SL, Wold B, Pachter L. Transcript assembly and quantification +by RNA-Seq reveals unannotated transcripts and isoform switching +during cell differentiation +Nature Biotechnology doi:10.1038/nbt.1621 + +Roberts A, Trapnell C, Donaghey J, Rinn JL, Pachter L. Improving RNA- +Seq expression estimates by correcting for fragment bias +Genome Biology doi:10.1186/gb-2011-12-3-r22 + +Roberts A, Pimentel H, Trapnell C, Pachter L. Identification of novel +transcripts in annotated genomes using RNA-Seq +Bioinformatics doi:10.1093/bioinformatics/btr355 + +Trapnell C, Hendrickson D,Sauvageau S, Goff L, Rinn JL, Pachter L +Differential analysis of gene regulation at transcript resolution with +RNA-seq +Nature Biotechnology doi:10.1038/nbt.2450 + +REFERENCES +--------------------------- +Cufflinks builds on many ideas, including some +proposed in the following papers: + +Ali Mortazavi, Brian A Williams, Kenneth McCue, Lorian Schaeffer and Barbara +Wold, "Mapping and quantifying mammalian transcriptomes by RNA-Seq",Nature +Methods, volume 5, 621 - 628 (2008) + +Hui Jiang and Wing Hung Wong, "Statistical Inferences for isoform expression", +Bioinformatics, 2009 25(8):1026-1032 + +Nicholas Eriksson, Lior Pachter, Yumi Mitsuya, Soo-Yon Rhee, Chunlin Wang, +Baback Gharizadeh, Mostafa Ronaghi, Robert W. Shafer, Niko Beerenwinkel, "Viral +population estimation using pyrosequencing", PLoS Computational Biology, +4(5):e1000074 diff --git a/academic/cufflinks/cufflinks.SlackBuild b/academic/cufflinks/cufflinks.SlackBuild new file mode 100644 index 0000000000000..04c2901a5992f --- /dev/null +++ b/academic/cufflinks/cufflinks.SlackBuild @@ -0,0 +1,107 @@ +#!/bin/sh + +# Slackware build script for cufflinks + +# Copyright 2013 Petar Petrov, petar.petrov@student.oulu.fi +# All rights reserved. +# +# Redistribution and use of this script, with or without modification, is +# permitted provided that the following conditions are met: +# +# 1. Redistributions of this script must retain the above copyright +# notice, this list of conditions and the following disclaimer. +# +# THIS SOFTWARE IS PROVIDED BY THE AUTHOR "AS IS" AND ANY EXPRESS OR IMPLIED +# WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF +# MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED. IN NO +# EVENT SHALL THE AUTHOR BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, +# SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES (INCLUDING, BUT NOT LIMITED TO, +# PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; LOSS OF USE, DATA, OR PROFITS; +# OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, +# WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR +# OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE, EVEN IF +# ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. + +# With help from Archlinux AUR and Gentoo: +# https://aur.archlinux.org/packages/cufflinks/ +# http://euscan.iksaif.net/package/sci-biology/cufflinks/ + +PRGNAM=cufflinks +VERSION=${VERSION:-2.1.1} +BUILD=${BUILD:-1} +TAG=${TAG:-_SBo} + +if [ -z "$ARCH" ]; then + case "$( uname -m )" in + i?86) ARCH=i486 ;; + arm*) ARCH=arm ;; + *) ARCH=$( uname -m ) ;; + esac +fi + +CWD=$(pwd) +TMP=${TMP:-/tmp/SBo} +PKG=$TMP/package-$PRGNAM +OUTPUT=${OUTPUT:-/tmp} + +if [ "$ARCH" = "i486" ]; then + SLKCFLAGS="-O2 -march=i486 -mtune=i686" + LIBDIRSUFFIX="" +elif [ "$ARCH" = "i686" ]; then + SLKCFLAGS="-O2 -march=i686 -mtune=i686" + LIBDIRSUFFIX="" +elif [ "$ARCH" = "x86_64" ]; then + SLKCFLAGS="-O2 -fPIC" + LIBDIRSUFFIX="64" +else + SLKCFLAGS="-O2" + LIBDIRSUFFIX="" +fi + +set -e + +rm -rf $PKG +mkdir -p $TMP $PKG $OUTPUT +cd $TMP +rm -rf $PRGNAM-$VERSION +tar xvf $CWD/$PRGNAM-$VERSION.tar.gz +cd $PRGNAM-$VERSION +chown -R root:root . +find -L . \ + \( -perm 777 -o -perm 775 -o -perm 750 -o -perm 711 -o -perm 555 \ + -o -perm 511 \) -exec chmod 755 {} \; -o \ + \( -perm 666 -o -perm 664 -o -perm 640 -o -perm 600 -o -perm 444 \ + -o -perm 440 -o -perm 400 \) -exec chmod 644 {} \; + +export EIGEN_CPPFLAGS="-I/usr/include/eigen3" + +CFLAGS="$SLKCFLAGS" \ +CXXFLAGS="$SLKCFLAGS" \ +./configure \ + --prefix=/usr \ + --libdir=/usr/lib${LIBDIRSUFFIX} \ + --sysconfdir=/etc \ + --disable-optim \ + --with-boost-libdir=/usr/lib${LIBDIRSUFFIX} \ + --localstatedir=/var \ + --mandir=/usr/man \ + --build=$ARCH-slackware-linux + +make +make install DESTDIR=$PKG + +find $PKG -print0 | xargs -0 file | grep -e "executable" -e "shared object" | grep ELF \ + | cut -f 1 -d : | xargs strip --strip-unneeded 2> /dev/null || true + +mkdir -p $PKG/usr/doc/$PRGNAM-$VERSION +cp -a \ + AUTHORS LICENSE README \ + $PKG/usr/doc/$PRGNAM-$VERSION +cat $CWD/$PRGNAM.SlackBuild > $PKG/usr/doc/$PRGNAM-$VERSION/$PRGNAM.SlackBuild +cat $CWD/References > $PKG/usr/doc/$PRGNAM-$VERSION/References + +mkdir -p $PKG/install +cat $CWD/slack-desc > $PKG/install/slack-desc + +cd $PKG +/sbin/makepkg -l y -c n $OUTPUT/$PRGNAM-$VERSION-$ARCH-$BUILD$TAG.${PKGTYPE:-tgz} diff --git a/academic/cufflinks/cufflinks.info b/academic/cufflinks/cufflinks.info new file mode 100644 index 0000000000000..0e90ccd4e4079 --- /dev/null +++ b/academic/cufflinks/cufflinks.info @@ -0,0 +1,10 @@ +PRGNAM="cufflinks" +VERSION="2.1.1" +HOMEPAGE="http://cufflinks.cbcb.umd.edu/" +DOWNLOAD="http://cufflinks.cbcb.umd.edu/downloads/cufflinks-2.1.1.tar.gz" +MD5SUM="3c1f34264122565b1697706a5f0fd242" +DOWNLOAD_x86_64="" +MD5SUM_x86_64="" +REQUIRES="eigen3 samtools" +MAINTAINER="Petar Petrov" +EMAIL="petar.petrov@student.oulu.fi" diff --git a/academic/cufflinks/slack-desc b/academic/cufflinks/slack-desc new file mode 100644 index 0000000000000..1a7d1f00545ba --- /dev/null +++ b/academic/cufflinks/slack-desc @@ -0,0 +1,19 @@ +# HOW TO EDIT THIS FILE: +# The "handy ruler" below makes it easier to edit a package description. +# Line up the first '|' above the ':' following the base package name, and +# the '|' on the right side marks the last column you can put a character in. +# You must make exactly 11 lines for the formatting to be correct. It's also +# customary to leave one space after the ':' except on otherwise blank lines. + + |-----handy-ruler------------------------------------------------------| +cufflinks: cufflinks (A reference-guided assembler for RNA-Seq experiments) +cufflinks: +cufflinks: Cufflinks assembles transcripts, estimates their abundances, +cufflinks: and tests for differential expression and regulation in RNA- +cufflinks: Seq samples. It accepts aligned RNA-Seq reads and assembles +cufflinks: the alignments into a parsimonious set of transcripts. +cufflinks: +cufflinks: Home: http://cufflinks.cbcb.umd.edu/ +cufflinks: References: /usr/doc/cufflinks-2.1.1/References +cufflinks: +cufflinks: |