diff options
author | Petar Petrov <slackalaxy@gmail.com> | 2018-07-08 19:42:08 +0100 |
---|---|---|
committer | David Spencer <idlemoor@slackbuilds.org> | 2018-07-08 19:42:08 +0100 |
commit | 4ce886af277886c629ab55efeb23b07874369c00 (patch) | |
tree | a85f77e8f01018ab9be721d1348b073ace28eba1 /academic | |
parent | 5c821121ece759ad907abbdf81c640c805eb197d (diff) |
academic/primer3: Updated for version 2.4.0.
Signed-off-by: David Spencer <idlemoor@slackbuilds.org>
Diffstat (limited to 'academic')
-rw-r--r-- | academic/primer3/gcc-7.patch | 22 | ||||
-rw-r--r-- | academic/primer3/man1/ntdpal.1 | 128 | ||||
-rw-r--r-- | academic/primer3/man1/ntthal.1 | 40 | ||||
-rw-r--r-- | academic/primer3/man1/oligotm.1 | 165 | ||||
-rw-r--r-- | academic/primer3/man1/primer3_core.1 | 90 | ||||
-rw-r--r-- | academic/primer3/primer3.SlackBuild | 32 | ||||
-rw-r--r-- | academic/primer3/primer3.info | 6 |
7 files changed, 467 insertions, 16 deletions
diff --git a/academic/primer3/gcc-7.patch b/academic/primer3/gcc-7.patch new file mode 100644 index 0000000000000..a92d0e77b7425 --- /dev/null +++ b/academic/primer3/gcc-7.patch @@ -0,0 +1,22 @@ +Author: Andreas Tille <tille@debian.org> +Last-Update: Sat, 26 Aug 2017 00:32:40 +0200 +Bug-Debian: https://bugs.debian.org/853621 +Description: Fix build with gcc-7 + +--- a/src/thal.c ++++ b/src/thal.c +@@ -426,12 +426,12 @@ thal(const unsigned char *oligo_f, + "Illegal type"); + o->align_end_1 = -1; + o->align_end_2 = -1; +- if ('\0' == oligo_f) { ++ if ('\0' == oligo_f[0]) { + strcpy(o->msg, "Empty first sequence"); + o->temp = 0.0; + return; + } +- if ('\0' == oligo_r) { ++ if ('\0' == oligo_r[0]) { + strcpy(o->msg, "Empty second sequence"); + o->temp = 0.0; + return; diff --git a/academic/primer3/man1/ntdpal.1 b/academic/primer3/man1/ntdpal.1 new file mode 100644 index 0000000000000..3f3cbfe228570 --- /dev/null +++ b/academic/primer3/man1/ntdpal.1 @@ -0,0 +1,128 @@ +'\" t +.\" Title: NTDPAL +.\" Author: [FIXME: author] [see http://docbook.sf.net/el/author] +.\" Generator: DocBook XSL Stylesheets v1.76.1 <http://docbook.sf.net/> +.\" Date: 11/30/2011 +.\" Manual: Primer3 User Manuals +.\" Source: ntdpal 1.1.4 +.\" Language: English +.\" +.TH "NTDPAL" "1" "11/30/2011" "ntdpal 1.1.4" "Primer3 User Manuals" +.\" ----------------------------------------------------------------- +.\" * Define some portability stuff +.\" ----------------------------------------------------------------- +.\" ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +.\" http://bugs.debian.org/507673 +.\" http://lists.gnu.org/archive/html/groff/2009-02/msg00013.html +.\" ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +.ie \n(.g .ds Aq \(aq +.el .ds Aq ' +.\" ----------------------------------------------------------------- +.\" * set default formatting +.\" ----------------------------------------------------------------- +.\" disable hyphenation +.nh +.\" disable justification (adjust text to left margin only) +.ad l +.\" ----------------------------------------------------------------- +.\" * MAIN CONTENT STARTS HERE * +.\" ----------------------------------------------------------------- +.SH "NAME" +ntdpal \- Provides Primer3\*(Aqs alignment functionality +.SH "SYNOPSIS" +.HP \w'\fBntdpal\fR\ 'u +\fBntdpal\fR [\-g\ \fIgval\fR] [\-l\ \fIlval\fR] [\-m\ \fImval\fR] [\-f1,\ f2,\ f3] [\-p] [\-s] [\-e] {\fIseq1\fR} {\fIseq2\fR} {\fImode\fR} +.SH "DESCRIPTION" +.PP +Ntdpal (NucleoTide Dynamic Programming ALignment) is a stand\-alone program that provides Primer3\*(Aqs alignment functionality (local, a\&.k\&.a\&. Smith\-Waterman, global, a\&.k\&.a\&. Needleman\-Wunsch, plus "half global")\&. +.SH "OPTIONS" +.PP +\fB\-g\fR \fIgval\fR +.RS 4 +\fIgval\fR +is a (positive) float (\&.01 precision) specifying penalty for creating a gap respectively (the penalties are subtracted from the output score) +.RE +.PP +\fB\-l\fR \fIval\fR +.RS 4 +\fIlval\fR +is a (positive) float (\&.01 precision) specifying penalty for lengthening a gap respectively (the penalties are subtracted from the output score) +.RE +.PP +\fB\-a\fR +.RS 4 +Causes the scoring matrix to be modified by dpal_set_ambiguity_codes\&. +.RE +.PP +\fB\-e\fR +.RS 4 +Causes the end position of the alignment in both sequences to be printed\&. Do not confuse with the \*(Aqe\*(Aq +\fImode\fR\&. +.RE +.PP +\fB\-f1, \-f2, \-f3\fR +.RS 4 +Force specific implementations\&. \-f2 forces use an implementation that might provide more informative error messages, possibly at the expense of some speed\&. +.RE +.PP +\fB\-h\fR +.RS 4 +Use a different scoring matrix: G and C matches = 3, A and T = 2, and mismatches = \-0\&.5\&. (The default scoring matrix assigns 1 to a match, and \-1 to a mismatch\&.) +.RE +.PP +\fB\-p\fR +.RS 4 +Causes the alignment to be displayed on stderr\&. +.RE +.PP +\fB\-s\fR +.RS 4 +causes +\fIonly\fR +the score to printed\&. +.RE +.PP +\fB\-m\fR \fImval\fR +.RS 4 +is the maximum allowed gap (default is 3)\&. +.RE +.PP +\fIseq1\fR and \fIseq2\fR +.RS 4 +are the sequences to be aligned\&. +.RE +.PP +\fImode\fR +.RS 4 +is one of +\fIg\fR, +\fIG\fR, +\fIl\fR, or +\fIL\fR +specifying a global, global end\-anchored, local, or local end\-anchored alignment respectively\&. For backward compatibility +\fIe\fR +is equivalent to +\fIG\fR\&. +.RE +.SH "REFERENCE" +.PP +Please cite Rozen, S\&., Skaletsky, H\&. "Primer3 on the WWW for general users and for biologist programmers\&." In S\&. Krawetz and S\&. Misener, eds\&. Bioinformatics Methods and Protocols in the series Methods in Molecular Biology\&. Humana Press, Totowa, NJ, 2000, pages 365\-386\&. +.SH "SEE ALSO" +.PP + +\fBprimer3_core\fR(1) +\fBoligotm\fR(1) +.SH "COPYRIGHT" +.br +Copyright \(co 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://jura.wi.mit.edu/rozen), Helen Skaletsky +.br +.PP +All rights reserved\&. On Debian\-based systems, please consult +/usr/share/doc/primer3/copyright +to read the licence of ntdpal\&. +.PP +This manual page was written by Charles Plessy <plessy@debian\&.org> for the +Debian(TM) +system (but may be used by others)\&. Permission is granted to copy, distribute and/or modify this document under the same terms as oligotm itself\&. + +.sp diff --git a/academic/primer3/man1/ntthal.1 b/academic/primer3/man1/ntthal.1 new file mode 100644 index 0000000000000..e3e5e8888a08f --- /dev/null +++ b/academic/primer3/man1/ntthal.1 @@ -0,0 +1,40 @@ +.\" DO NOT MODIFY THIS FILE! It was generated by help2man 1.43.3. +.TH NTTHAL "1" "December 2013" "2.3.6" "Primer3 User Manuals" +.SH NAME +ntthal \- Provides Primer3's alignment functionality based on nearest-neighbor thermodynamical approach +.SH DESCRIPTION +\fBntthal\fR is analogous to \fBntdpal\fR. Between two sequences, \fBntthal\fR finds +alignment/sec structure, that has the highest melting +temperature. Ntthal is based on nearest-neighbor thermodynamical +approach. +.SH SYNOPSIS +.B ntthal +\fIOPTIONS\fR oligo +.SH OPTIONS +\fB\-mv\fR monovalent_conc \- concentration of monovalent cations in mM, by default 50 mM +.PP +\fB\-dv\fR divalent_conc \- concentration of divalent cations in mM, by default 0 mM +.PP +\fB\-n\fR dNTP_conc \- concentration of deoxynycleotide triphosphate in mM, by default 0 mM +.PP +\fB\-d\fR dna_conc \- concentration of DNA strands in nM, by default 50 nM +.PP +\fB\-a\fR mode \- alignment type, END1, END2, ANY and HAIRPIN, by default ANY (when duplex) +.PP +\fB\-t\fR temp \- temperature at which duplex is calculated, by default 37C +.PP +\fB\-r\fR \- causes the alignment NOT to be displayed on stderr, _only_ Tm is printed +.PP +\fB\-maxloop\fR size \- the maximum size of secondary structures loops. +.IP +Default is 30 (this is maximum allowed length, currently). +.PP +\fB\-path\fR <path> \- the path to the thermodynamic parameter files +.PP +\fB\-s1\fR DNA_oligomer +.PP +\fB\-s2\fR DNA_oligomer +.SH AUTHORS +This manual page was created by Andreas Tille <tille@debian.org> using help2man +for Debian but can be freely used for any other purpose + diff --git a/academic/primer3/man1/oligotm.1 b/academic/primer3/man1/oligotm.1 new file mode 100644 index 0000000000000..ed9dcf7910d7b --- /dev/null +++ b/academic/primer3/man1/oligotm.1 @@ -0,0 +1,165 @@ +'\" t +.\" Title: OLIGOTM +.\" Author: [FIXME: author] [see http://docbook.sf.net/el/author] +.\" Generator: DocBook XSL Stylesheets v1.76.1 <http://docbook.sf.net/> +.\" Date: 11/30/2011 +.\" Manual: Primer3 User Manuals +.\" Source: oligotm 1.1.4 +.\" Language: English +.\" +.TH "OLIGOTM" "1" "11/30/2011" "oligotm 1.1.4" "Primer3 User Manuals" +.\" ----------------------------------------------------------------- +.\" * Define some portability stuff +.\" ----------------------------------------------------------------- +.\" ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +.\" http://bugs.debian.org/507673 +.\" http://lists.gnu.org/archive/html/groff/2009-02/msg00013.html +.\" ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +.ie \n(.g .ds Aq \(aq +.el .ds Aq ' +.\" ----------------------------------------------------------------- +.\" * set default formatting +.\" ----------------------------------------------------------------- +.\" disable hyphenation +.nh +.\" disable justification (adjust text to left margin only) +.ad l +.\" ----------------------------------------------------------------- +.\" * MAIN CONTENT STARTS HERE * +.\" ----------------------------------------------------------------- +.SH "NAME" +oligotm \- Prints oligo\*(Aqs melting temperature on stdout +.SH "SYNOPSIS" +.HP \w'\fBoligotm\fR\ 'u +\fBoligotm\fR [OPTIONS] {oligo} +.PP +where oligo is a DNA sequence of between 2 and 36 bases +.SH "DESCRIPTION" +.PP + +\fBoligotm\fR +prints the melting temperature of a given deoxyribonucleotide on the standard output\&. It is part of the oligotm library\&. +.SH "OPTIONS" +.PP +\fB\-mv\fR \fImonovalent_conc\fR +.RS 4 +Concentration of monovalent cations in mM, by default 50\ \&mM\&. +.RE +.PP +\fB\-dv\fR \fIdivalent_conc\fR +.RS 4 +Concentration of divalent cations in mM, by default 0\ \&mM\&. +.RE +.PP +\fB\-n\fR \fIdNTP_conc\fR +.RS 4 +Concentration of deoxynucleotide triphosphate in mM, by default 0\ \&mM\&. +.RE +.PP +\fB\-d\fR \fIdna_conc\fR +.RS 4 +Concentration of DNA strands in nM, by default 50\ \&nM\&. +.RE +.PP +\fB\-tp\fR \fI[0|1]\fR +.RS 4 +Specifies the table of thermodynamic parameters and the method of melting temperature calculation: +.sp +.RS 4 +.ie n \{\ +\h'-04'\(bu\h'+03'\c +.\} +.el \{\ +.sp -1 +.IP \(bu 2.3 +.\} +\fI0\fR +Breslauer et al\&., 1986 and Rychlik et al\&., 1990 (used by primer3 up to and including release 1\&.1\&.0)\&. This is the default, but +\fInot\fR +the recommended value\&. +.RE +.sp +.RS 4 +.ie n \{\ +\h'-04'\(bu\h'+03'\c +.\} +.el \{\ +.sp -1 +.IP \(bu 2.3 +.\} +\fI1\fR +Use nearest neighbor parameter from SantaLucia 1998\&. +\fIThis is the recommended value\fR\&. +.RE +.RE +.PP +\fB\-sc\fR \fI[0\&.\&.2]\fR +.RS 4 +Specifies salt correction formula for the melting temperature calculation: +.sp +.RS 4 +.ie n \{\ +\h'-04'\(bu\h'+03'\c +.\} +.el \{\ +.sp -1 +.IP \(bu 2.3 +.\} +\fI0\fR +Schildkraut and Lifson 1965, used by primer3 up to and including release 1\&.1\&.0\&. This is the default, but +\fInot\fR +the recommended value\&. +.RE +.sp +.RS 4 +.ie n \{\ +\h'-04'\(bu\h'+03'\c +.\} +.el \{\ +.sp -1 +.IP \(bu 2.3 +.\} +\fI1\fR +SantaLucia 1998\&. +\fIThis is the recommended value\fR\&. +.RE +.sp +.RS 4 +.ie n \{\ +\h'-04'\(bu\h'+03'\c +.\} +.el \{\ +.sp -1 +.IP \(bu 2.3 +.\} +\fI2\fR +Owczarzy et al\&., 2004\&. +.RE +.RE +.PP +\fB\-i\fR +.RS 4 +prints references to publications which were used for thermodynamic calculations\&. +.RE +.SH "REFERENCE" +.PP +Please cite Rozen, S\&., Skaletsky, H\&. "Primer3 on the WWW for general users and for biologist programmers\&." In S\&. Krawetz and S\&. Misener, eds\&. Bioinformatics Methods and Protocols in the series Methods in Molecular Biology\&. Humana Press, Totowa, NJ, 2000, pages 365\-386\&. +.SH "SEE ALSO" +.PP + +\fBprimer3_core\fR(1) +\fBntdpal\fR(1) +.SH "COPYRIGHT" +.br +Copyright \(co 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://jura.wi.mit.edu/rozen), Helen Skaletsky +.br +.PP +All rights reserved\&. On Debian\-based systems, please consult +/usr/share/doc/primer3/copyright +to read the licence of oligotm\&. +.PP +This manual page was written by Charles Plessy <plessy@debian\&.org> for the +Debian(TM) +system (but may be used by others)\&. Permission is granted to copy, distribute and/or modify this document under the same terms as oligotm itself\&. + +.sp diff --git a/academic/primer3/man1/primer3_core.1 b/academic/primer3/man1/primer3_core.1 new file mode 100644 index 0000000000000..aa99059dc6d8d --- /dev/null +++ b/academic/primer3/man1/primer3_core.1 @@ -0,0 +1,90 @@ +.\" Title: PRIMER3_CORE +.\" Author: +.\" Generator: DocBook XSL Stylesheets v1.73.2 <http://docbook.sf.net/> +.\" Date: 05/09/2008 +.\" Manual: Primer3 User Manuals +.\" Source: primer3_core 1.1.4 +.\" +.TH "PRIMER3_CORE" "1" "05/09/2008" "primer3_core 1.1.4" "Primer3 User Manuals" +.\" disable hyphenation +.nh +.\" disable justification (adjust text to left margin only) +.ad l +.SH "NAME" +primer3_core \- Designs primers for PCR +.SH "SYNOPSIS" +.HP 13 +\fBprimer3_core\fR [\-format_output] [\-strict_tags] [<\ \fIinput_file\fR] +.SH "DESCRIPTION" +.PP +primer3_core picks primers for PCR reactions, considering as criteria oligonucleotide melting temperature, size, GC content and primer\-dimer possibilities, PCR product size, positional constraints within the source sequence, and miscellaneous other constraints\&. +.PP +By default, primer3_core accepts input and produces output in Boulder\-io format, a pre\-XML text\-based input/output format for program\-to\-program data interchange format\&. The Boulder\-io format and the commands that primer3_core understands are described in the +\fIREADME\fR +file, which on Debian systems can be found in +\fI/usr/share/doc/primer3/\fR\&. +.SH "OPTIONS" +.PP +\fB\-format_output\fR +.RS 4 +Prints a more user\-oriented report for each sequence\&. +.RE +.PP +\fB\-strict_tags\fR +.RS 4 +primer3_core echoes and ignores any tags it does not recognize, unless the +\fB\-strict_tags\fR +flag is set on the command line, in which case primer3_core prints an error in the PRIMER_ERROR output tag, and prints additional information on stdout; this option can be useful for debugging systems that incorporate primer\&. +.RE +.sp +.it 1 an-trap +.nr an-no-space-flag 1 +.nr an-break-flag 1 +.br +Note +.PP +The old flag \-2x_compat is no longer supported\&. +.SH "EXIT STATUS CODES" +.sp +.RS 4 +\h'-04'\(bu\h'+03'0 on normal operation\&. +.RE +.sp +.RS 4 +\h'-04'\(bu\h'+03'\-1 under the following conditions: illegal command\-line arguments, unable to fflush stdout, unable to open (for writing and creating) a \&.for, \&.rev or \&.int file (probably due to a protection problem)\&. +.RE +.sp +.RS 4 +\h'-04'\(bu\h'+03'\-2 on out\-of\-memory\&. +.RE +.sp +.RS 4 +\h'-04'\(bu\h'+03'\-3 empty input\&. +.RE +.sp +.RS 4 +\h'-04'\(bu\h'+03'\-4 error in a "Global" input tag (message in PRIMER_ERROR)\&. +.RE +.SH "REFERENCE" +.PP +Please cite Rozen, S\&., Skaletsky, H\&. "Primer3 on the WWW for general users and for biologist programmers\&." In S\&. Krawetz and S\&. Misener, eds\&. Bioinformatics Methods and Protocols in the series Methods in Molecular Biology\&. Humana Press, Totowa, NJ, 2000, pages 365\-386\&. +.SH "SEE ALSO" +.PP + +\fBntdpal\fR(1) +\fBoligotm\fR(1) +.SH "COPYRIGHT" +Copyright \(co 1996,1997,1998,1999,2000,2001,2004,2006,2007,2008 Whitehead Institute for Biomedical Research, Steve Rozen (http://jura.wi.mit.edu/rozen), Helen Skaletsky +.br +.PP +All rights reserved\&. On Debian\-based systems, please consult +\fI/usr/share/doc/primer3/copyright\fR +to read the licence of primer3_core\&. +.PP +This manual page was written by Steffen Moeller +<moeller@debian\&.org> +for the +Debian(TM) +system (but may be used by others)\&. Permission is granted to copy, distribute and/or modify this document under the same terms as primer3_core itself\&. + +.sp diff --git a/academic/primer3/primer3.SlackBuild b/academic/primer3/primer3.SlackBuild index 8e60d3fe02fca..d3ae1b399c1b2 100644 --- a/academic/primer3/primer3.SlackBuild +++ b/academic/primer3/primer3.SlackBuild @@ -2,7 +2,7 @@ # Slackware build script for primer3 -# Copyright 2011-2016 Petar Petrov slackalaxy@gmail.com +# Copyright 2011-2018 Petar Petrov slackalaxy@gmail.com # All rights reserved. # # Redistribution and use of this script, with or without modification, is @@ -23,13 +23,13 @@ # ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. PRGNAM=primer3 -VERSION=${VERSION:-2.3.7} +VERSION=${VERSION:-2.4.0} BUILD=${BUILD:-1} TAG=${TAG:-_SBo} if [ -z "$ARCH" ]; then case "$( uname -m )" in - i?86) ARCH=i486 ;; + i?86) ARCH=i586 ;; arm*) ARCH=arm ;; *) ARCH=$( uname -m ) ;; esac @@ -40,8 +40,8 @@ TMP=${TMP:-/tmp/SBo} PKG=$TMP/package-$PRGNAM OUTPUT=${OUTPUT:-/tmp} -if [ "$ARCH" = "i486" ]; then - SLKCFLAGS="-O2 -march=i486 -mtune=i686" +if [ "$ARCH" = "i586" ]; then + SLKCFLAGS="-O2 -march=i586 -mtune=i686" LIBDIRSUFFIX="" elif [ "$ARCH" = "i686" ]; then SLKCFLAGS="-O2 -march=i686 -mtune=i686" @@ -69,6 +69,9 @@ find -L . \ \( -perm 666 -o -perm 664 -o -perm 640 -o -perm 600 -o -perm 444 \ -o -perm 440 -o -perm 400 \) -exec chmod 644 {} \; +# Thanks to Debian for the patch +patch -p1 -i $CWD/gcc-7.patch + cd ./src # Use our CFLAGS @@ -81,8 +84,8 @@ CFLAGS="$SLKCFLAGS" \ CXXFLAGS="$SLKCFLAGS" \ make -# This is recommended, but the tests take a really long time. -# Be patient if you uncomment the next line. +# This is recommended, but the tests take a really long time. Be patient +# if you uncomment the line below. # make test install -D -m755 primer3_core $PKG/usr/bin/primer3_core @@ -97,18 +100,21 @@ ln -s primer3_core primer32_core # Copy configuration files and settings to /usr/share cd $TMP/$PRGNAM-$VERSION mkdir -p $PKG/usr/share/$PRGNAM -cp -a src/primer3_config \ - primer3_v1_1_4_default_settings.txt \ - primer3web_v0_4_0_default_settings.txt \ - primer3web_v3_0_0_default_settings.txt \ - $PKG/usr/share/$PRGNAM +cp -a settings_files src/primer3_config $PKG/usr/share/$PRGNAM + +# Copy the man pages from Debian +mkdir -p $PKG/usr/man/man1 +cp $CWD/man1/* $PKG/usr/man/man1 find $PKG -print0 | xargs -0 file | grep -e "executable" -e "shared object" | grep ELF \ | cut -f 1 -d : | xargs strip --strip-unneeded 2> /dev/null || true +find $PKG/usr/man -type f -exec gzip -9 {} \; +for i in $( find $PKG/usr/man -type l ) ; do ln -s $( readlink $i ).gz $i.gz ; rm $i ; done + mkdir -p $PKG/usr/doc/$PRGNAM-$VERSION cp -a \ - src/release_notes.txt COPYING.txt example primer3_manual.htm \ + src/{release_notes.txt,primer3_manual.htm} LICENSE example README.md \ $PKG/usr/doc/$PRGNAM-$VERSION cat $CWD/$PRGNAM.SlackBuild > $PKG/usr/doc/$PRGNAM-$VERSION/$PRGNAM.SlackBuild cat $CWD/References > $PKG/usr/doc/$PRGNAM-$VERSION/References diff --git a/academic/primer3/primer3.info b/academic/primer3/primer3.info index 11f9865090f05..861eb56e58729 100644 --- a/academic/primer3/primer3.info +++ b/academic/primer3/primer3.info @@ -1,8 +1,8 @@ PRGNAM="primer3" -VERSION="2.3.7" +VERSION="2.4.0" HOMEPAGE="http://primer3.sourceforge.net/" -DOWNLOAD="http://sourceforge.net/projects/primer3/files/primer3/2.3.7/primer3-2.3.7.tar.gz" -MD5SUM="c6b89067bf465e62b6b1fd830b5b4418" +DOWNLOAD="http://sourceforge.net/projects/primer3/files/primer3/2.4.0/primer3-2.4.0.tar.gz" +MD5SUM="aed6546bdfb60652cd7eba8d51eae8cc" DOWNLOAD_x86_64="" MD5SUM_x86_64="" REQUIRES="" |